Real-Time multiplex PCR kits - Multiplexing that serves the clinical need
Syndromically grouped Real-Time PCR
Fast-track diagnostics produces syndromically grouped real-time PCR multiplex kits that serve the clinical need, this is important because a clinical examination alone cannot easily distinguish between viral, bacterial or other infections – PCR can!
Imagine a typical scenario where a person feels ill and goes to the doctor for a diagnosis. If the doctor is concerned about the patient’s symptoms and overall presentation further investigation will be necessary. A sample is taken and a test is often ordered from the lab based on what the Doctor expects to be the most likely pathogenic cause of the illness. If the test comes back negative, a second test must be ordered, if it also comes back negative a third or more, until a positive result is found.
This cycle of repeat testing is bad for the patient, frustrating, wastes time, reagents and money and the patient remains untreated waiting for a diagnosis – possibly getting sicker. Worse yet, the patient could be prescribed unnecessary medication before the test results are confirmed, like antibiotics for a viral illness.
Now imagine the same scenario BUT the doctor can order a test from the lab covering all probable pathogenic causes – virus, bacteria, fungi or parasite - at the same time, in one run – this is syndromic Real-Time PCR multiplex testing. It saves time, resources, reagents and money, but most importantly it is better for the patient because a fast clinical diagnosis reduces morbidity and the risk of mortality - particularly in high risk immuno-compromised hospital cases like transplant patients, or the elderly and the young.
Fast-track diagnostics manufactures one of the largest ranges of syndromically grouped Real-Time PCR multiplexes available – covering all the major disease groups like respiratory infections, gastroenteritis, sexually transmitted infections, fevers and rash, meningitis, infections of the immunosuppressed and tropical fevers.
The severity of viruses, viral load and antibiotic resistance
Data shows that the pathogenic load is often related to the severity of the illness, for example with pneumonia, so where an understanding of the viral load or level of infection is critical, we offer quantitative tests too. The quantification is possible using either quantification standards supplied with the kit or a cycler specific formula.
Another benefit of rapid diagnosis with Fast-track diagnostic kits is that it can help avoid the development of antibiotic resistance by allowing pathogen specific treatment - as many as 75% of all antibiotic doses are prescribed for acute respiratory tract infections. Of these, most are caused by viruses not bacteria.
Finally, viruses are often the cause of far more severe infections than is generally realised, for example:
- Pneumonia in ITU is usually assumed to be due to bacteria but in fact can be caused by many viruses such as rhinoviruses, flu, RSV and human metapneumovirus.
- 75% of all community gastroenteritis is caused by viruses.
- Neonatal sepsis can be caused by parechoviruses and adenoviruses.
- EBV and adenovirus are routine, but often unexpected, causes of post transplant sepsis.